LC & LC-MS Series

Fundamentals of HPLC

This course introduces the essential concepts every scientist needs to use liquid chromatography effectively—from how HPLC systems work and why reversed-phase separations are standard, to gradients, quantitation, separation chemistry, and practical maintenance tips. By the end, you’ll have a clear understanding of core HPLC principles and greater confidence in applying them in the lab.
A female scientist documenting sample results in a laboratory | Fundamentals of HPLC course from Analytical Training Solutions
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  • 5+ Hours

    of Video Content

    Learners

    Quizzes

    Modules

    Videos
    LC & LC-MS Series

    Course Description

    ‘Fundamentals of HPLC’ covers the essentials that every scientist needs in order to make effective use of liquid chromatography instrumentation. 

    By taking this online course you will acquire a good understanding of the fundamentals of HPLC and get a better knowledge of the key concepts involved.

    LC & LC-MS Series

    Course Highlights

    • The basics of the chromatographic process

    • Terms and definitions of chromatography

    • How HPLC systems work

    • Practical tips on preventive maintenance

    • Principles of separation chemistry

    • Why “reversed phase” is the standard separation technique

    • The meaning of “gradients” and why they are used

    • How to get the best quantitation

    About the instructor

    Tom Jupille

    Tom has been a practising chromatographer for more than 40 years, during which he has written more than 30 papers on chromatography and related subjects. He worked primarily in gas chromatography in the late ‘60s, switching to thin-layer chromatography in the early ‘70s and then to HPLC and ion chromatography in late ‘70s.
    His career has focused on instrument and column development and user support, providing a broad foundation of practical experience to call on as an instructor. He is moderator of the popular Chromatography Forum on-line chromatography discussion group.

    Preview This Course for Free

    Experience the first module before enrolling.
    Click the video activity in the first module below to get started—no purchase required.