LC & LC-MS Series

Practical HPLC for Biopharmaceuticals

This course demystifies HPLC for both small-molecule and biopolymer separations, giving you a clear, practical understanding of the techniques used across biopharmaceutical workflows. You’ll learn core principles, instrument fundamentals, key separation modes, quantitation, and troubleshooting—equipping you with a versatile analytical toolkit for characterizing and quantifying complex samples.
A graphic of a pharmaceutical capsule on top of a computer chip | Graphic for "Practical HPLC for Biopharmaceuticals" course from Analytical Training Solutions

  • Solo or Team

    Subscriptions Available
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  • 12+ Hours

    of Video Content

    Learners

    Quizzes

    Modules

    Videos
    LC & LC-MS Series

    Course Description

    “Practical HPLC for Biopharmaceuticals” explains chromatography in practical terms from the ground up.

    Although “small molecule” and “biopolymer” separations have traditionally been considered as separate activities, analysts in the biopharmaceutical industry regularly have to deal with both. Fortunately, the underlying principles of chromatography apply equally well in both situations when interpreted appropriately. 
    LC & LC-MS Series

    Course Highlights

    • The basics of isocratic and gradient HPLC

    • Reversed phase and ion exchange HPLC

    • HPLC equipment – from reservoirs/pumps to tubing/injectors to columns and detectors

    • Understanding and overcoming common column problems

    • Alternative modes: normal phase, HILIC and size exclusion

    • Quantitation

    • UHPLC

    • Performance qualification

    • Troubleshooting and diagnostics

    About the instructor

    Tom Jupille

    Tom has been a practising chromatographer for more than 40 years, during which he has written more than 30 papers on chromatography and related subjects. He worked primarily in gas chromatography in the late ‘60s, switching to thin-layer chromatography in the early ‘70s and then to HPLC and ion chromatography in late ‘70s.
    His career has focused on instrument and column development and user support, providing a broad foundation of practical experience to call on as an instructor. He is moderator of the popular Chromatography Forum on-line chromatography discussion group.

    Preview This Course for Free

    Experience the first module before enrolling.
    Click the video activity in the first module below to get started—no purchase required.