LC-MS/MS for Chromatographers
This course offers a practical introduction to operating and optimizing LC-MS/MS systems. You’ll learn how ions are generated and analyzed, how quadrupole instruments work, and how to set up reliable qualitative and quantitative methods—from calibration and tuning to sample prep and validation—so you can use LC-MS/MS confidently in everyday analysis.
-
Solo or Team
Subscriptions Available
See Team Options -
10+ Hours
of Video Content
Learners
Quizzes
Modules
Videos
LC & LC-MS Series
Course Description
With the introduction of the mass spectrometer (MS) as a practical detector for a high-performance liquid chromatograph (LC or HPLC) in the early 1990s, LC-MS began to be used for routine applications. One form of LC-MS uses a tandem MS (LC-MS/MS), that has become the go-to instrument for quantitative analysis of drugs in biological matrices in the pharmaceutical laboratory. As they have become more widely used and understood, LC-MS and LC-MS/MS have found their way into environmental applications, synthetic chemistry, protein analysis,
and many other areas. LC-MS/MS has moved from a technique available only to skilled experts in both HPLC and MS to a routine tool usable much as any other HPLC detector.
You will find that this introductory course in LC-MS/MS will give you a good foundation of understanding the technique and how to use it in your daily work. We start out with an overview of different MS techniques, and focus in the majority of the course on the quadrupole mass spectrometer. You will learn how MS works, how ions are produced and fragmented. Sections focus on qualitative, as well as quantitative analysis. Each part of the process, calibration, tuning, sample preparation, HPLC method development, and MS method development are covered. Many tricks and techniques of practical use are presented.
and many other areas. LC-MS/MS has moved from a technique available only to skilled experts in both HPLC and MS to a routine tool usable much as any other HPLC detector.
You will find that this introductory course in LC-MS/MS will give you a good foundation of understanding the technique and how to use it in your daily work. We start out with an overview of different MS techniques, and focus in the majority of the course on the quadrupole mass spectrometer. You will learn how MS works, how ions are produced and fragmented. Sections focus on qualitative, as well as quantitative analysis. Each part of the process, calibration, tuning, sample preparation, HPLC method development, and MS method development are covered. Many tricks and techniques of practical use are presented.
LC & LC-MS Series
Course Highlights
John Dolan
John Dolan is considered to be the one of the world’s experts in HPLC. He has written more than 300 user-oriented articles on HPLC troubleshooting over the last 30 years in addition to more than 100 peer-reviewed technical articles on HPLC and related techniques.
His three books (co-authored with Lloyd Snyder), Troubleshooting HPLC Systems, Introduction to Modern Liquid Chromatography (3rd edn), and High-Performance Gradient Elution, are standard references on thousands of desks around the world. He has taught HPLC training classes around the world to more than 10,000 students.
Write your awesome label here.
Preview This Course for Free
Experience the first module before enrolling.
Click the video activity in the first module below to get started—no purchase required.
Click the video activity in the first module below to get started—no purchase required.
